TITLE:
Diversity of mushrooms in Dry Dipterocarp forest at Phuphan National Park, Sakon Nakhon Province
AUTHORS:
Pithak Wongchalee, C. Pukahute
KEYWORDS:
The Diversity; Mushroom; The Dry Dipterocarp Forest; The Phuphan National Park
JOURNAL NAME:
Natural Science,
Vol.4 No.12A,
December
31,
2012
ABSTRACT: The purposes of this study were, 1) to study the variety of mushrooms grown in the Dry Dipterocarp forest during the year 2008-2009 by Releve method, 2) to study the relationship between ShoreasiamensisMiq. And Ectomycorrhizal of the Amanitaceae and the Belotaceae families, and 3) study the sequencesof DNAsinsome types of mushrooms in the Amanitaceae and the Balotaceae families by the PCR method. The findings of the study were as thefollowings: First, they were totally 34 types of mush-rooms found in Dry Dipterocarp forest at the Phuphan National Park during the studying period, 2008- 2009. There were 26 types found in both years Amanita hemibapha subsp. javanica Corner & Bas, A. princeps Corner & Bas, A. umbrinolutea (Secr. ex Gillet) Bataille, Termi-tomycesmicro- carpus (Berk. & Br.) R. Heim, T. perforans Heim, T. striatus f. griseus Heim, Boletellusananas (M. A. Curtis) Murrill, B. grisei-purpureus Cor., B. edulis Bull. ex Fr., B. luridus Schaeffer ex Fr., AlpovatrappeiFogel, Can-tharelluscibarius Fries, Craterellusaureus Berk. & Curis, Astraeushygrometricus (Pers.) Morgan, Lactariusaquifluus Peck, L. glaucescens Crossl., L. piperatus (Scop. ex Fr.) S. F. Gray, L. vellereus (Fr.) Fr., L. virescens Fr., R. densifolia (Secr.) Gill, R. emetica (Schaeff. & Fr.) S.F. Gray., R. xelempelina (Schaeff.) Fr., R. foetens (Pers.) Fr., R. rosacea (Pers. ex Secr.) Fries, R. violeipes Quél., R. virescens (Schaeff.) Fries. and those found only in 2009 were Bo-letuscurtisii M. A. Curtis, B. nobilis Peck, RussulaalboareolataHongo, R. cyanoxantha (Schaeff. ex Secr.) Fr. The mushrooms that had been found had significantly relations to the surrounding physical conditions. Second, there was a relationship between the Shoreasiamensis Miq. and Amanita princeps Cor.& Bas., Amanita hemibapha (Berk. & Br.) Sacc. subsp.javanica. Cor. & Bas., Boletus chrysenteron Bull., Boletus griseipurpureus Cor. and Heimiellarestipora (Pat & Bek.) Boedijn. at the intensity of spores 500, 5000, 50000 per each. From the SPSS for ANOVA analysis, the Shoreasiamensis Miq. had no relations to Amanita princeps Cor. & Bas., Amanita hemibapha (Berk. & Br.) Sacc. subsp. javanica. Cor. & Bas., Boletus chrysenteron Bull., Boletus grisei-purpureus Cor. and Heimiellarestipora (Pat & Bek.) Boedijn. in height and circumference growths of Shoreasiamensis Miq. seedlings at the intensity of spores 500, 5000 and 50,000 per each Shoreasiamensis Miq. at 95% level of confidence. Finally, by using the BLASTN computer program toexamine, compare and separate the differences 4 types of mushrooms’ DNA in the Amanitaceae and the Boletaceae families subjected to the PCR method, there were 4 findings i.e. 1342 DNA sbp. of Amanita princeps, 880 DNAs pb. of Boletusnobitis Peck, 1381 DNAs pb. of Boletus edulis Bull. exFr and 758 DNAs bp. of Heimiellaresipora (Pat & Baker).