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A. Mellmann, J. Cloud, T. Maier, U. Keckevoet, I. Ramminger, P. Iwen, J. Dunn, G. Hall, D. Wilson, P. Lasala, M. Kostrzewa and D. Harmsen, “Evaluation of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry in Comparison to 16S rRNA Gene Sequencing for Species Identification of Nonfermenting Bacteria,” Journal of Clinical Microbiology, Vol. 46, No. 6, 2008, pp. 1946-1954. doi:10.1128/JCM.00157-08

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• TITLE: Mass Spectrometric Identification of Propionibacterium Isolates Requires Database Enrichment

  AUTHORS: Sophie Edouard, Carine Couderc, Didier Raoult, Pierre-Edouard Fournier

  KEYWORDS: Propionibacterium; Propionibacterium acnes; MALDI-TOF; Mass Spectrometry

  JOURNAL NAME: Advances in Microbiology, Vol.2 No.4, December 27, 2012

  ABSTRACT: Propionibacterium species are mostly environmental bacteria, some being commensal of mammals including humans, and sometimes pathogenic. These bacteria are poorly identified using routine laboratory methods. Recently, Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) has emerged as a rapid and efficient method to identify bacterial species. We evaluated the use of MALDI-TOF-MS for identification of all validated Propionibacterium species. Only four of the 15 tested reference strains (26.7%) were correctly identified at the species level, and P. acnes, the most common human pathogenic species was not identified. When applying MALDI-TOF-MS to 48 P. acnes strains, only 18.7% were correctly identified, suggesting an intraspecific variability of proteic profiles among Propionibacterium strains. However, by enriching the Bruker database with spectra from five of these strains and re-testing the other 43 strains against this new database, 93.0% were correctly identified. Our study demonstrates that MALDI-TOF-MS may be used for the identification of Propionibacterium isolates but requires a database enrichment in spectra from additional isolates.