TITLE:
Comparison of energy-related isoenzymes between production and racing Arabian camels
AUTHORS:
Mohammed S. AL-Harbi, Sayed A. M. Amer
KEYWORDS:
Malate Dehydrogenase; Malic Enzyme; Racing Camel; Production Camel; Electrophoresis
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.3 No.8,
December
27,
2012
ABSTRACT: Native polyacrylamide gel electrophoresis have been applied for the analyses of alkaline phosphatase (ALP), malate dehydrogenase (Mdh) and malic (ME) isoenzymes in Arabian camel for racing and production. Two fractions for each of these isoenzymes have been recorded in the studied breeds. ALP showed very weak patterns without remarkable difference between the two breeds and this is an indication to that the samples used were healthy and being from the same age. The cytosolic Mdh-1 and ME-1 have been recorded in both camel breeds with high intensity. The mitochondrial Mdh-2 and ME-2 have been recorded with small intensity in production breeds commonly. The present data indicate the necessity of the mitochondrialMdh-2 for energy production in racing breed and the responsibility of the cytosolic Mdh-1 for lipogenesis and energy production in both breeds. We therefore may assume that the appearance of both Mdh forms is necessary for both energy and lipid production in the production breeds while Mdh-1 was useful as bioenergetic enzyme necessary for racing. The different expressions are indications of the difference in the physiological adaptations of both camel breeds and are not for a systematic value.