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A. Boyum, “Isolation of Mononuclear Cells and Granulocytes from Human Blood. Isolation of Monuclear Cells by One Centrifugation, and of Granulocytes by Combining Centrifugation and Sedimentation at 1 g,” Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 97, 1968, pp. 77-89.

has been cited by the following article:

• TITLE: Transcriptional Expression Profile of Toll Like Receptor 1 - 10 mRNA in Bovine Peripheral Mononuclear Cells in Response to Foot and Mouth Disease Antigens

  AUTHORS: Sathish Gaikwad, Sowmya Kumar, Thimmareddy Prashanth, Golla Rama Reddy, Veluvarthy Sanyasi Suryanarayana, Hosur Joyappa Dechamma

  KEYWORDS: Innate Immunity; FMDV; DNA Vaccine; TLR Genes

  JOURNAL NAME: Advances in Microbiology, Vol.2 No.4, December 25, 2012

  ABSTRACT: Toll like receptors are the primary component of innate immune response. The comparison of expression of innate immune genes TLR 1 - 10 was carried out by real time PCR by quantifying mRNA in bovine PBMCs. The bovine PBMCs in vitro stimulated with FMD infectious virus lead to up regulation of TLR 2, 3, 4 and down regulation of TLR 9. But stimulation with DNA vaccine carryingVP1 genes of FMDV leads to up regulation of TLR 2 only. Difference in the TLR gene expression by the virus and DNA vaccine, may be related to the outcome of the vaccine and carrier or persistent state of the animal which is seen in FMDV.