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A. Lucca, G. Gentilini, S. Lopez-Silva and A. Soldarini, “Simultaneous Determination of Human Plasma Levels of Four Selective Serotonin Reuptake Inhibitors by High-Performance Liquid Chromatography,” Therapeutic Drug Monitoring, Vol. 22, No. 3, 2000, pp. 271-276. doi:10.1097/00007691-200006000-00007

has been cited by the following article:

• TITLE: Quantitative Determination of Fluoxetine in Pharmaceuticals and Plasma Samples Using Bromatometric Method

  AUTHORS: Jasmin Shah, Muhammad Rasul Jan, Muhammad Naeem Khan, Sadaf Durrani

  KEYWORDS: Fluoxetine; Bromatometric Method; Methyl Orange; Spectrophotometry

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.3 No.12, December 25, 2012

  ABSTRACT: Asensitive and simple spectrophotometric method has been developed for quantitative determination of fluoxetine using bromatometric method. The method is based on the addition of measured excess amount of bromate-bromide mixture to fluoxetine in hydrochloric acid medium. The residual bromine was determined by reacting with a fixed amount ofmethyl orange and absorbance was measured at 505 nm. The amount of bromine reacted corresponds to the amount of fluoxetine. Linear relationship between absorbance and fluoxetine concentration was found and Beer’s law was obeyed in the concentration range of 0.4 - 12 μg·mL–1. The molar absorptivity was found to be 3.8 × 104 L·mol–1·cm–1. The limit of detection and limit of quantification was calculated and found to be 0.32 μg·mL–1 and 1.0 μg·mL–1respectively. The common excipients were investigated for their interferences effect in the assay. The validity of the developed method was checked through recoveries studies and successfully applied to the determination of fluoxetine in bulk powder, pharmaceutical formulations and spiked human plasma samples. The percent recoveries were found to be in the range of 97.0% - 101.0% for pharmaceutical formulations and from 97.0% - 99.0% for spiked human plasma.