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M. H. Jaffe, G. Hosgood, H. W. Taylor, S. C Kerwin, C. S. Hedlund, M. K. Lopez, J. R. Davidson, D. M. Miller and M. Paranjpe, “Immunohistochemical and Clinical Evaluation of p53 in Canine Cutaneous Mast Cell Tumors,” Veterinary Pathology, Vol. 37, No. 1, 2000, pp. 40-46. doi:10.1354/vp.37-1-40

has been cited by the following article:

• TITLE: Evaluation of Collection and Distribution of Samples for Histological, Stereological Analysis and Cell Culture of Canine Mast Cell Tumors

  AUTHORS: Samanta Rios Melo, Thaís Andrade Costa-Casagrande, Julia Maria Matera

  KEYWORDS: Mast Cell Tumor; Oncologic Surgery; Canine; Tumor Collection; Stereological Analysis

  JOURNAL NAME: Open Journal of Veterinary Medicine, Vol.2 No.4, December 14, 2012

  ABSTRACT: Objective: To collect high quality, representative tissue material from tumors and manage its distribution to different laboratories. Design: Prospective controlled study. Animals: Thirty-six dogs with mast cell tumors. Procedures: The samples were submitted for the following analyses: stereology; histopathology; cell culture; breakdown for cytogenetic analysis of chromosomes (based on the Boxer breed published genome); Cell lysis for Real Time PCR and quantification of gene expression of CX 43, 32 and E-cadherin in canine mast cells. Results: Cytogenetic chromosome analysis, 90.9% of the samples were considered to be of good quality. For gene expression quantification of CX 43, 32 and E-cadherin in canine mast cell tumors (MCT), 95.5% of samples were considered to be of good quality. Conclusions and Clinical Relevance: We seek to assess the importance of surgical collection and post-surgical tissue preparation on laboratory testing by collecting surgical material appropriately to allow accurate diagnosis and reliable clinical prognosis and minimize errors caused by inadequate preparation of samples.