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B. Séguin, N. F. Leibman, V. S. Bregazzi, G. K. Ogilvie, B. E. Powers, W. S. Dernell, M. J. Fettman and S. J. Withrow, “Clinical Outcome of Dogs with Grade II Mast Cell Tumors Treated with Surgery Alone: 55 Cases (1996-1999),” Journal of the American Veterinary Medical Association, Vol. 218, No. 7, 2001, pp. 1120-1123. doi:10.2460/javma.2001.218.1120

has been cited by the following article:

• TITLE: Evaluation of Collection and Distribution of Samples for Histological, Stereological Analysis and Cell Culture of Canine Mast Cell Tumors

  AUTHORS: Samanta Rios Melo, Thaís Andrade Costa-Casagrande, Julia Maria Matera

  KEYWORDS: Mast Cell Tumor; Oncologic Surgery; Canine; Tumor Collection; Stereological Analysis

  JOURNAL NAME: Open Journal of Veterinary Medicine, Vol.2 No.4, December 14, 2012

  ABSTRACT: Objective: To collect high quality, representative tissue material from tumors and manage its distribution to different laboratories. Design: Prospective controlled study. Animals: Thirty-six dogs with mast cell tumors. Procedures: The samples were submitted for the following analyses: stereology; histopathology; cell culture; breakdown for cytogenetic analysis of chromosomes (based on the Boxer breed published genome); Cell lysis for Real Time PCR and quantification of gene expression of CX 43, 32 and E-cadherin in canine mast cells. Results: Cytogenetic chromosome analysis, 90.9% of the samples were considered to be of good quality. For gene expression quantification of CX 43, 32 and E-cadherin in canine mast cell tumors (MCT), 95.5% of samples were considered to be of good quality. Conclusions and Clinical Relevance: We seek to assess the importance of surgical collection and post-surgical tissue preparation on laboratory testing by collecting surgical material appropriately to allow accurate diagnosis and reliable clinical prognosis and minimize errors caused by inadequate preparation of samples.