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Al Mansur, M.A.Z., Haque, M.S., Nasiruddin, K.M. and Hossain, M.S. (2009) In vitro propagationof bitter gourd (Momoridica charantia L.) from nodal and root segments. Plant Tissue Culture and Biotechnology, 19, 45-52.

has been cited by the following article:

• TITLE: The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

  AUTHORS: Nasir S. A. Malik, Jose L. Perez, Madhurababu Kunta

  KEYWORDS: Momordica charantia; Polyamines; Rooting; Defoliation; Propagation

  JOURNAL NAME: Agricultural Sciences, Vol.3 No.7, November 23, 2012

  ABSTRACT: The study was conducted to investigate the optimal hormone treatment for rooting in bitter melon and the effect of defoliation on rooting and polyamine levels. Commercial preparation (diluted 1:10 and 1:20) gave extensive rooting within five days after treatment. The presence of leaf with the stem cutting was necessary for optimal rooting as defoliation drastically reduced rooting even when the rooting time was extended from 5 to 10 days. The presence of leaf also promoted the levels of putrescine three days after the hormone treatment just at the time of root emergence. Cuttings that did not have leaf did not show an increase in putrescine levels and did not produce roots. Spermidine levels also increased in the cuttings that rooted but there was no change in the levels of spermine in the cuttings that rooted. It appears that putrescine and spermidine play a role in regulating rooting in bitter melon cuttings. The vegetative propagation conditions described show that it is a practically viable technique for small growers to produce large number of plantlets at the farm for selection and cultivation.