TITLE:
Acceleration of Hericium erinaceum mycelial growth in submerged culture using yogurt whey as an alternative nitrogen source
AUTHORS:
Chikako Asada, Ryosuke Okumura, Chizuru Sasaki, Yoshitoshi Nakamura
KEYWORDS:
Hericium Erinaceum; Mycelia; Submerged Culture; Yogurt Whey
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.3 No.7,
November
20,
2012
ABSTRACT: The effects of various carbon sources and their initial concentrations on mycelia production by Hericium erinaceum were investigated by determining the dry cell weight (DCW) and β-glucan content of mycelia in submerged culture. Glucose and xylose were superior carbon sources for promoting mycelial growth resulting in mycelial concentrations of 3.99 g/L and 4.01 g/L, respectively; glucose was the best carbon source in terms of productivity (0.44 g/L/day). Experiments were also performed using yogurt whey as an alternative nitrogen source for submerged cultivation of H. erinaceum mycelia, and DCW and β-glucan content were compared with those with chemical nutrient medium. When whey was used as a nitrogen source, DCW and total amount of β-glucan were 2.3- and 2.8-fold higher, respectively, than that with chemical nutrient medium. Thus, whey appears to be an alternative nitrogen source for promoting H. erinaceum mycelial growth.