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S. Caillet, M. Millette, D. Dussault, F. Shareck and M. Lacroix, “Effect of Gamma Radiation on Heat Shock Protein Expression of Four Foodborne Pathogens,” Journal of Applied Microbiology, Vol. 105, No. 5, 2008, pp. 1384-1391. doi:10.1111/j.1365-2672.2008.03891.x

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• TITLE: Induction of DnaK upon γ-Irradiation at High and Low Rates in Escherichia coli

  AUTHORS: Linda Saucier, Claudine Dube, Anthony Guernec, Fadia Naim

  KEYWORDS: DnaK; Chaperone; Irradiation; Bacterial Stress Response; Efficacy of Antimicrobials

  JOURNAL NAME: Food and Nutrition Sciences, Vol.3 No.10, October 12, 2012

  ABSTRACT: DnaK is implicated in protein folding, repair and degradation. Its protective role during heat shock is well documented and many other stresses can also induce its production. Using a competitive ELISA, intracellular DnaK concentrations were determined in Escherichia coli ATCC 25922 exposed to a γ-irradiation dose of 0.3 KGy applied either at high (8 × 10-2 KGy/min) or low rates (3 × 10-3 KGy/min) and with or without a recuperation period of 22 h at 37℃ post-treatment. All four irradiation treatments reduced cell counts similarly and significantly compared to the control (P