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S. S. Tai, C. G. Lin, M. H. Wu and T. S. Chang, “Evaluation of Depigmenting Activity by 8-Hydroxydaidzein in Mouse B16 Melanoma Cells and Human Volunteers,” International Journal of Molecular Sciences, Vol. 10, No. 10, 2009, pp. 4257-4266. doi:10.3390/ijms10104257

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• TITLE: Qualitative and Quantitative Estimation of Hydroquinone in Skin Whitening Cosmetics

  AUTHORS: Saima Siddique, Zahida Parveen, Zeeshan Ali, Muhammad Zaheer

  KEYWORDS: Hydroquinone; HPLC; Whitening Cosmetics; Thin Layer Chromatography

  JOURNAL NAME: Journal of Cosmetics, Dermatological Sciences and Applications, Vol.2 No.3, September 18, 2012

  ABSTRACT: Hydroquinone has been used for decades as a skin lightening agent. Its use in cosmetics has been banned as a result of skin problems including contact dermatitis and ochronosis. A total of 22 samples of different skin whitening cosmetics were collected from local market. They were analyzed by using thin layer chromatography and HPLC for qualitative and quantitative determination of their hydroquinone contents. The hydroquinone was extracted from samples by using 96% ethanol and was subjected to TLC analysis. Eleven out of 22 samples were found to contain hydroquinone. The HPLC analysis showed the concentration of hydroquinone ranged from 0.002% to 0.092% in the cosmetic samples.