TITLE:
Motility and Functional State of the Membrane of Caprine Capacitated Spermatozoa under Different Chemical Agents
AUTHORS:
A. Soberano Martínez, J. Herrera Camacho, J. C. Segura Correa
KEYWORDS:
Spermatozoa; Capacitation; Caprine; Chemical Agents
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.2 No.3,
September
28,
2012
ABSTRACT: The objective of this study was to test the use of a commercial extender (Triladyl) as a diluent in caprine semen refrigerated at 15℃, using caffeine (CF), heparin (HP), synthetic oviductal fluid (SOF) andtriladyl (TRY) as capacitating chemical agents at different times. Twenty ejaculates of caprine semen were collected using an artificial vagina. The ejaculates were diluted and refrigerated by three days. Evaluated the progressive motility (PM) and the functional state of the sperm plasma membrane trough fluorescent CTC staining, counting 200 spermatic cells (non-capacitated spermatozoa NCS, capacitated CS and reacting spermatozoa REA) of caprine in two capacitating agents: CAF and HEP; one culture medium:SOF and a commercial extender: TRY at 60, 120, 180 and 240 min of incubation, during 24, 48 and 72 hs. PM was high under TRY, and CS was high under the HEP treatment. TRY could be an alternative to capacitate caprine spermatozoa, keeping PM for a longer time than HEP or CAF.