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A. Hussein, N. Al-Wadei, T. Takahashi and H. M. Schuller, “Theophylline Stimulates cAMP-Mediated Signaling Associated with Growth Regulation in Human Cells from Pulmonary Adenocarcinoma and Small Airway Epithelia,” International Journal of Oncology, Vol. 27, No. 1, 2005, pp. 155-160.

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• TITLE: The Melanin Biosynthesis Stimulating Compounds Isolated from the Fruiting Bodies of Pleurotus citrinopileatus

  AUTHORS: Tian-Xiao Meng, Chao-Feng Zhang, Tomofumi Miyamoto, Hiroya Ishikawa, Kuniyoshi Shimizu, Shoji Ohga, Ryuichiro Kondo

  KEYWORDS: Pleurotus citrinopileatus; myo-Inositol; D-Mannitol; Melanin Stimulating Activity; White Hair-Preventive Agent; Tanning Agent

  JOURNAL NAME: Journal of Cosmetics, Dermatological Sciences and Applications, Vol.2 No.3, September 17, 2012

  ABSTRACT: In the course to find a stimulating compound for melanin biosynthesis, which should be useful for a gray and a white hair-preventive agent or tanning agent, we evaluated the effects of the methanol extract from mushroom of Pleurotus citrinopileatus on melanin production in B16 melanoma cells without theophylline. Activity-guided fractionation led to isolate myo-inositol (3) and D-mannitol (4) as the stimulating compounds on melanin production in B16 melanoma cells. Also, ergosterol (1), uracil (2), and D-glucose (5) have been isolated from the methanol extract of P. citrinopileatus and showed no effect on melanin production in B16 melanoma cells. These results indicated that myo-inositol (3) and D-mannitol (4) are potential candidates that could be useful such as a gray and a white hair-preventive agent or tanning agent.