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Pennetier, S., Uzbekova, S., Perreau, C., Papiller, P., Mermillod, P. and Dalbiès-Tran, R. (2004) Spatio-temporal expression of germ cell marker genes MATER, ZAR1, GFD9, BMP15, and VASA in adult bovine tissues, oocytes, and preimplantation embryos. Biology of Reproduction, 71, 1359-1366. doi:10.1095/biolreprod.104.030288

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• TITLE: High pregnancy and implantation rates can be obtained with preincubation of oocytes before insemination in IVF and ICSI procedures

  AUTHORS: Javier García-Ferreyra, Pedro Valdivieso, Milton Zambrano, Jorge Carpio

  KEYWORDS: Oocytes; Embryo Development; Maturation; Blastocyst; ART

  JOURNAL NAME: Health, Vol.4 No.9, September 28, 2012

  ABSTRACT: Purpose: Evaluate the effect of preincubation of oocytes prior to IVF or ICSI cycles with embryo transfer at blastocyst stage. Methods: Retrospective non randomized study based on secondary analysis of data. Setting: Laboratory of Assisted Reproduction at the Alcivar Hospital. Patients: One hundred-eighteen cycles of IVF and ICSI were analyzed in the present study. The evaluated groups were formed for those patients whose oocytes, after retrieval, were inseminated at 1-3 h (Group I) or 4-6 h (Group II). Results: There was no difference in fertilization rate (83.6% and 78.1%), Day 3 cleavage rate (95.1% and 97.1%), and blastocyst formation (31.1% and 39.1%) for groups I and II respectively. Clinical pregnancy rates (PR: 53.0% vs 22.9%) and implantation rates (IR: 38.1% vs 13.0%) were significantly higher in group II versus group I, respectively (P