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A. D. Kloosterman, B. Budowle and M. Daselaar, “PCR-Amplification and Detection of the Human DIS80 VNTR Locus. Amplification Conditions, Population Genetics and Application in Forensic Analysis,” International Journal of Legal Medicine, Vol. 105, No. 5, 1993, pp. 257-264. doi:10.1007/BF01370382

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• TITLE: Assessment of Genetic Diversity of Moroccan Cultivated Almond (Prunus dulcis Mill. DA Webb) in Its Area of Extreme Diffusion, Using Nuclear Microsatellites

  AUTHORS: Abdelali Elhamzaoui, Ahmed Oukabli, Jamal Charafi, Mohiéddine Moumni

  KEYWORDS: Almond; Genetic Diversity; Polymorphism; Spatial Genetic Structure; Prunus dulcis; Microsatellites; SSR

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.9, September 27, 2012

  ABSTRACT: Assessment of genetic diversity of Moroccan cultivated almond (Prunus dulcis Mill.) grown from seed and cultivated at four eco-geographical regions was performed using 16 nuclear SSRs. 238 alleles were detected with an average of 14.88 alleles per locus, ranging from 4 (locus BPPCT027) to 24 (locus CPSCT018). The size of alleles ranged from 84 bp (locus UDP96-003) to 253 bp (locus UDP96-018). A high genetic diversity of the local almonds is apparent and structured into three major clusters (Oasis cluster, High and Anti Atlas cluster, and Middle Atlas cluster). Compared to the Mediterranean genetic pools, from the East to West, the genetic diversity tends to be limited in Morocco which is the area of its extreme diffusion.