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G. F. L. Ames, C. S. Mimura and V. Shyamala, “Bacterial Periplasmic Permeases Belong to a Family of Transport Proteins Operating from Escherichia coli to Human: Traffic ATPases,” FEMs Microbiological Review, Vol. 75, No. 4, 1990, pp. 429-446. doi:10.1016/0378-1097(90)90691-I

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• TITLE: Genetic Transformation Studies on Avocado Cultivar “Hass” (Persea americana)

  AUTHORS: Muhammad F. Ahmed, Arumugam S. Kantharajah, Paul Holford

  KEYWORDS: Avocado; Persea; Binary Vectors; GUS Reporter

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.9, September 26, 2012

  ABSTRACT: The use of traditional breeding for improvement of avocado cultivars is time consuming, hence other methods such as genetic transformation by Agrobacterium is indispensable to adopt. The strain GV3850/pBI121gave best transformation outcome compared to five other binary vectors (AGL1/pCGP904; AGL1/pBI121; GV3850/pCGP904; LBA4404/pCG-P904 and LBA4404/pBI121) under different pH and acetosyringone concentrations. The optimal condition for reliable transformation was by using 200 μM acetosyringone and a pH of 5.2. Transformed embryonic shoots co-cultivated with GV3850/pBI121 were tested using the histochemical x-gluc assay. Further analysis was conducted by polymerase chain reaction using specific primers for the reporter gene (GUS).