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M. M. Lulsdorf, H. Rempel, J. A. Jackson, D. S. Baliski and S. L. A. Hobbs, “Optimizing the Production of Transformed Pea (Pisum sativum L.) Callus Using Disarmed Agrobacterium tumefaciens Strains,” Plant Cell Reports, Vol. 9, No. 9, 1991, pp. 479-483. doi:10.1007/BF00232100

has been cited by the following article:

• TITLE: Genetic Transformation Studies on Avocado Cultivar “Hass” (Persea americana)

  AUTHORS: Muhammad F. Ahmed, Arumugam S. Kantharajah, Paul Holford

  KEYWORDS: Avocado; Persea; Binary Vectors; GUS Reporter

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.9, September 26, 2012

  ABSTRACT: The use of traditional breeding for improvement of avocado cultivars is time consuming, hence other methods such as genetic transformation by Agrobacterium is indispensable to adopt. The strain GV3850/pBI121gave best transformation outcome compared to five other binary vectors (AGL1/pCGP904; AGL1/pBI121; GV3850/pCGP904; LBA4404/pCG-P904 and LBA4404/pBI121) under different pH and acetosyringone concentrations. The optimal condition for reliable transformation was by using 200 μM acetosyringone and a pH of 5.2. Transformed embryonic shoots co-cultivated with GV3850/pBI121 were tested using the histochemical x-gluc assay. Further analysis was conducted by polymerase chain reaction using specific primers for the reporter gene (GUS).