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A. Tanaka and Y. Hase, “Establishment of Ion Beam Technology for Breeding,” In: Q. Y. Shu, Ed., Induced Plant Mutations in the Genomics Era, Food and Agriculture Organization of the United Nations, 2009, pp. 243-246.

has been cited by the following article:

• TITLE: An Improved System for Shoot Regeneration from Stem Explants of Lombardy Poplar (Populus nigra L. var. italica Koehne)

  AUTHORS: Kamal Kanti Biswas, Takeshi Mohri, Satoshi Kogawara, Yoshihiro Hase, Yutaka Oono

  KEYWORDS: Lombardy Poplar; Shoot Regeneration; Stem Explants; Auxin and Benzylaminopurine; Radiation Sensitivity

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.9, September 26, 2012

  ABSTRACT: We developed a system for the regeneration of Lombardy poplar (Populus nigra L. var. italica) shoots from internodal stem explants. Using this system, shoots regenerated from 87% of the stem explants placed on Murashige and Skoog (MS) medium supplemented with 0.1 mg/L indole-3-acetic acid and 0.5 mg/L benzylaminopurine without undergoing callus formation. About 80% of the in vitro regenerated shoots developed roots on MS medium supplemented with 0.5 mg/L indole-3-butyric acid and 0.02 mg/L 1-naphthylacetic acid. Well-rooted seven-to eight-week-old regenerated plants could be transferred to soil for further growth and the survival rate of such plants after three weeks was 88%. The protocol presented here is simple and economical because it does not rely on pre-incubation in callus induction medium or repeated subculture in shoot induction medium containing trans-zeatin, an expensive substance. The in vitro regeneration system presented here could be used for evaluation of radiation sensitivity for Lombardy poplar tissues.