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A. Tanaka, N. Shikazono and Y. Hase, “Studies on Biological Effects of Ion Beams on Lethality, Molecular Nature of Mutation Rate, and Spectrum of Mutation Phenotype for Mutation Breeding in Higher Plants,” Journal of Radiation Research, Vol. 51, No. 3, 2010, pp. 223-233. doi:10.1269/jrr.09143

has been cited by the following article:

• TITLE: An Improved System for Shoot Regeneration from Stem Explants of Lombardy Poplar (Populus nigra L. var. italica Koehne)

  AUTHORS: Kamal Kanti Biswas, Takeshi Mohri, Satoshi Kogawara, Yoshihiro Hase, Yutaka Oono

  KEYWORDS: Lombardy Poplar; Shoot Regeneration; Stem Explants; Auxin and Benzylaminopurine; Radiation Sensitivity

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.9, September 26, 2012

  ABSTRACT: We developed a system for the regeneration of Lombardy poplar (Populus nigra L. var. italica) shoots from internodal stem explants. Using this system, shoots regenerated from 87% of the stem explants placed on Murashige and Skoog (MS) medium supplemented with 0.1 mg/L indole-3-acetic acid and 0.5 mg/L benzylaminopurine without undergoing callus formation. About 80% of the in vitro regenerated shoots developed roots on MS medium supplemented with 0.5 mg/L indole-3-butyric acid and 0.02 mg/L 1-naphthylacetic acid. Well-rooted seven-to eight-week-old regenerated plants could be transferred to soil for further growth and the survival rate of such plants after three weeks was 88%. The protocol presented here is simple and economical because it does not rely on pre-incubation in callus induction medium or repeated subculture in shoot induction medium containing trans-zeatin, an expensive substance. The in vitro regeneration system presented here could be used for evaluation of radiation sensitivity for Lombardy poplar tissues.