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P. Ghosh, N. B. Pamment, and W. R. B. Martin, “Simultaneous Saccharification and Fermentation of Cellulose: β-D-gluodidase Activity and Ethanol Inhibition of Cellulases,” Enzyme and Microbial Technology, Vol. 4, No. 6, 1982, pp. 425-430. doi:10.1016/0141-0229(82)90075-8

has been cited by the following article:

• TITLE: Ethanol Tolerance in Aspergillus niger and Escherichia coli Phytase

  AUTHORS: Edward J. Mullaney, Kandan Sethumadhavan, Stephanie Boone, Abul H. J. Ullah

  KEYWORDS: Phytase; Ethanol; DDGS; Fermentation; Aspergillus niger

  JOURNAL NAME: Advances in Microbiology, Vol.2 No.3, September 25, 2012

  ABSTRACT: Despite yeast having its own native phytase, the high levels of phytate found in DDGS, a byproduct of ethanol (ETOH) fermentation, suggest that its activity is diminished in the presence of ETOH. Ethanol, a product of grain fermentation, is known to inactivate several hydrolytic enzymes but its effect on phytases is relatively unknown. In this study, two phytases, Aspergillus niger (PhyA) and Escherichia coli (AppA2), were tested for ETOH tolerance. The E. coli phytase displayed greater ethanol tolerance over fungal phytase in the 5% to 10% range. However, ETOH inactivation was found to be reversible for both the enzymes. These differences in ETOH tolerance do suggest that there is a potential to achieve higher ETOH tolerance in phytases by 'structure-function' studies to lower phytic acid levels in DDGS and for other applications.