TITLE:
Identification of a T-cell epitope in the Staphylococcus aureus Panton-Valentine LukS-PV component
AUTHORS:
Eric L. Brown, Keri Csencsits Smith, Maria Gabriela Bowden
KEYWORDS:
Staphylococcus; Cell Mediated Immunity; Panton-Valentine Leukocidin; T Cells
JOURNAL NAME:
Open Journal of Immunology,
Vol.2 No.3,
September
21,
2012
ABSTRACT: We previously observed the elicitation of a significant delayed-type hypersensitivity response to the Panton-Valentine leukocidin (PVL) LukS-PV subunit following subcutaneous immunization (Brown et al., Clinical Microbiology and Infection, 2009, 156: 156-164). A LukS-PV-specific cell line (LST) was screened for proliferative responses against a panel of 25 amino acid-long peptides spanning the length of LukS-PV (amino acids 29-312). This analysis demonstrated that stimulation of LST with LukS-PV resulted in significant proliferative responses and adoptive transfer of LST into na?ve mice conferred a LukS-PV-specific DTH response following challenge. Challenge of mice adoptively transferred with LST with peptides 7 (149-173), 8 (169-193) and 14 (289-312) also elicited a measurable DTH response suggesting that these peptides contained T cell epitopes.