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M. Z. Vasquez, “Recommendations for Safety Testing with the in Vivo Comet Assay,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 747, No. 1, 2012, pp. 142-156. doi:10.1016/j.mrgentox.2012.05.002

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• TITLE: The Concurrence of Hypercholesterolemia and Aging Promotes DNA Damage in Apolipoprotein E-Deficient Mice

  AUTHORS: Sara P Dalboni, Bianca P Campagnaro, Clarissa L Tonini, Elisardo C Vasquez, Silvana S Meyrelles

  KEYWORDS: DNA Damage; Oxidative Stress; apoE Knockout Mice; Aging; Blood Cells

  JOURNAL NAME: Open Journal of Blood Diseases, Vol.2 No.3, September 4, 2012

  ABSTRACT: Recent evidence shows that increased oxidative stress and aging contribute to DNA damage in various cardiovascular diseases such as lipid disorders and atherosclerosis. In the present study, we used the comet assay to evaluate the influ- ence of aging on DNA damage in whole blood cells from apolipoprotein E-deficient (apoE?/?) mice and compared the results to those found in cells from wild-type C57BL/6 (C57) mice. Using the alkaline comet assay and fluorescent ethidium bromide staining, DNA damage was analyzed in the peripheral whole blood (5 μL) cells that were isolated from either young (8-week-old) and elderly (72-week-old) apoE?/? mice or from age-matched C57 mice. The levels of total plasma cholesterol were approximately 6-fold higher in apoE?/? mice of both ages compared to C57 mice. Elderly apoE?/? mice showed significantly higher levels of DNA damage (19%) compared to elderly C57 mice (8%, p