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T. J. White, T. Bruns, S. Lee and J. W. Taylor, “Amplification and Direct Sequencing of Fungal Ribosomal RNA genes for Phylogenetics,” In: M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, Eds., PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc., New York, 1990, pp. 315-322.

has been cited by the following article:

• TITLE: Fungal Isolate “KMI” Is a New Type of Orchid Mycorrhizal Fungus

  AUTHORS: Tomoko Matsubara, Motohito Yoneda, Takaaki Ishii

  KEYWORDS: KMI; Orchid Mycorrhizal Fungus; Paphiopedilum thailandense; Protein Analysis, Ribosomal DNA Sequence

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.8, August 31, 2012

  ABSTRACT: We analyzed an isolate of fungus that seems to be a new type orchid mycorrhizal fungus, “KMI (refers to Kyoto-Ma- tsubara-Ishii)”, obtained from the roots of Paphiopedilum thailandense Fowl. KMI has hyphal branching into right angle similar to Rhizoctonia-like fungi, but its spore formation resembled Nectoria, which is known as a teleomorph of Fusarium rather than Rhizoctonia. Its ribosomal DNA sequences of 18S and ITS have no similarity with any known fungal species. Proteins in molecular weight of 53 and 24 kDa, which are common to mycorrhizal fungi, were detected in KMI. When KMI was inoculated onto orchids, however it didn’t form pelotons or coils, but the hyphae in the root tissues were observed and the tissues were not decayed. No significant symptoms of Rhizoctonia or Fusarium disease, however, were developed on tomato and cucumber plants. The evidences suggest that KMI is a new type of orchid mycorrhizal fungus.