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M. A. Helm and V. Hemleben, “Characterization of a New Prominent Satellite DNA of Cucumis metuliferus and Differential Distribution of Satellite DNA in Cultivated and Wild Species of Cucumis and in Related Genera of Cucurbitaceae,” Euphytica, Vol. 94, No. 2, 1997, pp. 219-226. doi:10.1023/A:1002976408213

has been cited by the following article:

• TITLE: Genetic Diversity and Species Identification of Cultivar Species in Subtribe Cucumerinae (Cucurbitaceae) Using RAPD and SCAR Markers

  AUTHORS: Juthaporn Saengprajak, Piyaporn Saensouk

  KEYWORDS: Cucumerinae; Cucurbitaceae; Genetic Diversity; Random Amplified Polymorphic DNA (RAPD); Sequence Characterized Amplified Region (SCAR); Species Identification

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.8, August 31, 2012

  ABSTRACT: DNA polymorphism in the cultivar species; Cucumis sativus L., C. melo L. and Benincasa hispida Cogn. of subtribe Cucumerinae (Cucurbitaceae) in the four northeastern provinces of Thailand was examined by using RAPD technique. Twenty 10-mer primers were produced 212 RAPD fragments, ranging from approximately 120 to 2531 bp. The genetic similarities were estimated from banding profiles using a NTSYS* version 2.1 as a basis for dendrogram construction via the UPGMA method. Cluster analysis divided the taxa under study into 2 clades. Moreover, a RAPD marker: Cm (OPJ11700) was specified to C. melo, and this marker was converted into sequence characterized amplified region (SCAR) marker: Cm (SCJ11516). A pair of sequence-specific primer of clones Cm (OPJ11700) amplified a distinct single band of the same size as the RAPD clones. The SCAR marker was developed successfully to identify C. melo genotype.