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Cai, Y.A., Murphy, J.T., Wedemayer, G.J. and Glazer, A.N. (2001) Recombinant phycobili-proteins: Recombinant C-phycocyanins equipped with affinity tags, oligomerization, and biospecific recognition domains. Analytical Biochemistry, 290, 186-204.

has been cited by the following article:

• TITLE: Large scale preparation of phycobiliproteins from Porphyra yezoensis using co-precipitation with ammonium sulfate

  AUTHORS: Chuner Cai, Chunxia Li, Shuxian Wu, Qing Wang, Ziye Guo, Peimin He

  KEYWORDS: Porphyra yezoensis; Phycoerythrin; Phycocyanin; Ammonium Sulfate Precipitation; Hydroxylapatite

  JOURNAL NAME: Natural Science, Vol.4 No.8, August 29, 2012

  ABSTRACT: The purpose of this study was to extract phyco-biliproteins (PBP) from Porphyra yezoensis using an optimized procedure and further establish a large scale process for protein production. According to our previous experiences on the extraction of PBP, salting out methods, e.g. ammonium sulfate precipitation, worked more efficiently than isoelectric precipitation, differential centrifugation or ultrafiltration. Thus, we chose ammonium sulfate to coprecipitate PBP in crude solution. After four times of precipitation followed by one time of high speed centrifugation, the maximum purity of crude phycoerythrin and phycocyanin reached 1.94 (A565/A280) and 0.85 (A615/A280), with a yield of 0.50% and 0.37%, respectively. A total of 0.94 mg phycoerythrin and 0.54 mg phycocyanin with purity of more than 3.2 were obtained from 1 g dried P. yezoensis after additional chromatography. We further scaled up the frozen dried P. yezoensis from 20 g to 400 g, with 1295 mg phycoerythrin and 593 mg phycocyanin obtained.