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H. Lei, M. Ting, L. Dan, L. Feng-Lai, L. Ru-Lin, et al., “Optimization of Nutrient Component for Diesel Oil Degradation by Rhodococcus erythropolis,” Marine Pollution Bulletin, Vol. 56, No. 10, 2008, pp. 1714-1718. doi:10.1016/j.marpolbul.2008.07.007

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• TITLE: Factorial Designs Application to Study Enhanced Bioremediation of Soil Artificially Contaminated with Weathered Bonny Light Crude Oil through Biostimulation and Bioaugmentation Strategy

  AUTHORS: Samuel E. Agarry, Oladipupo O. Ogunleye

  KEYWORDS: Bioremediation; Box-Behnken Designs; Crude Oil; Second-Order Quadratic Regression Model

  JOURNAL NAME: Journal of Environmental Protection, Vol.3 No.8, August 21, 2012

  ABSTRACT: The objective of this study was designed to evaluate the effects of biostimulation and bioaugmentation amendment agents (NPK fertilizer, Tween 80 and mixed culture) on the bioremediation of tropical soil samples artificially contaminated with Weathered Bonny Light Crude Oil (WBLCO). Response Surface Methodology (RSM) with Box Behnken Design (BBD) was used with three levels and three factors of NPK fertilizer (2 - 6 g), Tween 80 (5 - 15 mg/l) and mixed culture (0.5 - 1.5 g/l) as independent variables and WBLCO removal as dependent variable (response) in a six weeks remediation period. The results showed that the rate of WBLCO removal generally increased with increase in the amount of NPK fertilizer, Tween 80 and mixed culture (biomass), respectively. A statistically significant (P