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Wainwright, P.E., Huang, Y.S., Coscina, D.V., Levesque, S. and McCutcheon, D. (1994) Brain and behavioral effects of dietary n ? 3 deficiency in mice: A three generational study. Developmental Psychobiology, 27, 467-487. doi:10.1002/dev.420270705

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• TITLE: Visualization of decreased docosahexaenoic acid in the hippocampus of rats fed an n – 3 fatty acid-deficient diet by imaging mass spectrometry

  AUTHORS: Shu Taira, Michio Hashimoto, Kazunori Saito, Osamu Shido

  KEYWORDS: Docosahexaenoic Acid; Imaging Mass Spectrometry; Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry; Hippocampus

  JOURNAL NAME: Journal of Biophysical Chemistry, Vol.3 No.3, August 28, 2012

  ABSTRACT: The present study employed an imaging mass spectrometry (IMS) method to evaluate the effect of dietary n – 3 fatty acids on the fatty acid composition in rat brain. Rats were divided into two groups and fed either an n – 3 fatty acid-deficient or adequate diet. We determined the decreased n – 3 fatty acids in the hippocampus of rats fed an n – 3 fatty acid-deficient diet compared to the control. IMS visualization was achieved at a resolution of 100 m in rat brain, and showed decreased docosahexaenoic acid (DHA)-containing phosphatidyl choline (PC) or phosphatidyl ethanolamine (PE) in the hippocampus of rats fed an n – 3 fatty acid-deficient diet.