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Araque, A., Parpura, V., Sanzgiri, R.P. and Haydon, P.G. (1999) Tripartite synapses: Glia, the unacknowledged partner. Trends in Neurosciences, 22, 208-215. doi:10.1016/S0166-2236(98)01349-6

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• TITLE: Effectiveness of gelatin solidification for unfrozen preservation and transportation of astrocytes

  AUTHORS: Nobuko Ishibashi, Shinichi Sotome, Kazuyuki Nakajima, Akio Shimizu

  KEYWORDS: Cell preservation; Gelatin; Astrocytes; Unfrozen Preservation; Transportation

  JOURNAL NAME: Advances in Biological Chemistry, Vol.2 No.3, August 22, 2012

  ABSTRACT: We herein studied a non-cryogenic cell preservation and transportation method. We found that astrocytes can be preserved in an unfrozen state at 4℃ (without medium exchange), while maintaining good condition in comparison with cryopreservation for the periods of 5 - 7 days. The gelatin solidification method can also prevent decreases in survival rate by preventing damage to astrocytes during transportation. Therefore, the gelatin solidification method at 4℃ may be a useful choice for short-term preservation and transportation.