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Polge. C., Smith, A.U. and Parkes, A.S. (1949) Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature, 164, 666. doi:10.1038/164666a0

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• TITLE: Effectiveness of gelatin solidification for unfrozen preservation and transportation of astrocytes

  AUTHORS: Nobuko Ishibashi, Shinichi Sotome, Kazuyuki Nakajima, Akio Shimizu

  KEYWORDS: Cell preservation; Gelatin; Astrocytes; Unfrozen Preservation; Transportation

  JOURNAL NAME: Advances in Biological Chemistry, Vol.2 No.3, August 22, 2012

  ABSTRACT: We herein studied a non-cryogenic cell preservation and transportation method. We found that astrocytes can be preserved in an unfrozen state at 4℃ (without medium exchange), while maintaining good condition in comparison with cryopreservation for the periods of 5 - 7 days. The gelatin solidification method can also prevent decreases in survival rate by preventing damage to astrocytes during transportation. Therefore, the gelatin solidification method at 4℃ may be a useful choice for short-term preservation and transportation.