TITLE:
Effectiveness of gelatin solidification for unfrozen preservation and transportation of astrocytes
AUTHORS:
Nobuko Ishibashi, Shinichi Sotome, Kazuyuki Nakajima, Akio Shimizu
KEYWORDS:
Cell preservation; Gelatin; Astrocytes; Unfrozen Preservation; Transportation
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.2 No.3,
August
22,
2012
ABSTRACT: We herein studied a non-cryogenic cell preservation and transportation method. We found that astrocytes can be preserved in an unfrozen state at 4℃ (without medium exchange), while maintaining good condition in comparison with cryopreservation for the periods of 5 - 7 days. The gelatin solidification method can also prevent decreases in survival rate by preventing damage to astrocytes during transportation. Therefore, the gelatin solidification method at 4℃ may be a useful choice for short-term preservation and transportation.