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Flores, M.V., Naraghi, K., Engasser, J.M. and Halling, P.J. (2002) Influence of glucose solubility and dissolution rate on the kinetics of lipase catalyzed synthesis of glucose laurate in 2-methyl 2-butanol. Biotechnology and Bioengineering, 78, 814-820. doi:10.1002/bit.10263

has been cited by the following article:

• TITLE: Lipase-catalyzed regioselective synthesis of palmitolyglucose ester in ionic liquids

  AUTHORS: Jintian Liang, Wentian Zeng, Pingjia Yao, Yuanan Wei

  KEYWORDS: Ionic Liquids; 6-O-Palmitolyglucose Ester; Lipase; Palmitic Acid Vinyl Ester; Glucose

  JOURNAL NAME: Advances in Biological Chemistry, Vol.2 No.3, August 22, 2012

  ABSTRACT: Candida antarctica lipase B (CAL-B) was used as a catalyst in the synthesis of palmitolyglucose ester in the ionic liquids, 1-butyl-3-methylimidazolium triflu- oromethanesulfonate ([Bmim][TfO]), with glucose as a substrates and palmitic acid vinyl ester as the acyl donor. The effect of substrate ratio, lipase content, and temperature on the activity and stability of lipase was studied. The reaction conditions in [Bmim][TfO] re- sulting in the highest yield of the sugar ester were a temperature of 50?C, enzyme concentration of 50 mg/ mL, and a molar ratio of glucose/vinyl palmitate of 1:3. The major reaction product was purified and char- acterized by FT-IR, HPLC, MS and NMR, as being 6-O-palmitolyglucose ester. The advantages of ionic liquid vs. organic solvent were noted.