TITLE:
Cytotoxicity of Pristine C60 Fullerene on Baby Hamster Kidney Cells in Solution
AUTHORS:
Shufang Liu, Haijie Liu, Zhijuan Yin, Kai Guo, Xibao Gao
KEYWORDS:
C60; Cell; Cytotoxicity; Fullerene; Toxicity;
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.3 No.3,
July
4,
2012
ABSTRACT: Research on biological effects of C60 and its derivatives is one of the hotspots in the area of biological effects of nanomaterials. Compared with surface-modified C60 derivatives, reports on the biological effects of unmodified pristine C60 are relatively less. This work aimed to investigate the interaction between baby hamster kidney cells and pristine C60 in solution. The C60 suspension was prepared using solvent exchange method and characterized by UV spectrophotometry, electronic transmission microscopy and dynamic light scattering techniques. The baby hamster kidney cells were incubated with different concentrations of C60 suspensions, and light microscopy, cell counting kit 8 assay, and acridine orange staining were used to observe the cell growth and morphology. The results showed that C60 could ihibit the cell growth and induce cell apoptosis with a dose-effect relationship. C60 might enter cells and the possible way it enters cells were also proposed.