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S. Xie, M. A. Furjanic, J. J. Ferrara, N. R. McAndrew, E. L. Ardino, A. Ngondara, Y. Bernstein, K. J. Thomas, E. Kim, J. M. Walker, S. Nagar, S. J. Ward and R. B. Raffa, “The Endocannabinoid System and Rimonabant: A New Drug With a Novel Mechanism of Action Involving Cannabi-noid CB1 Receptor Antagonism—Or Inverse Agonism—As Potential Obesity Treatment and Other Therapeutic Use,” Journal of Clinical Pharmacy and Therapeutics, Vol. 32, No. 3, 2007, pp. 209-231.
doi:10.1111/j.1365-2710.2007.00817.x
has been cited by the following article:
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TITLE:
Development of a Behavioral and Imaging Model of Feeding in Planarians
AUTHORS:
Robert B. Raffa, Christopher S. Tallarida, Shan R. Patel, Scott M. Rawls, Evgeny Krynetskiy
KEYWORDS:
Feeding; Imaging; Fluorescence; GFP; Planarians
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.3 No.3,
July
5,
2012
ABSTRACT: Excess weight is a major risk factor for type-2 diabetes, cardiovascular disease, and other comorbidities. Animal models of feeding provide insight into the problem and provide a means for the discovery and evaluation of pharmacotherapeutic treatment. Mammalian models are the most commonly used, but recently non-mammalian models have been utilized (e.g., C. elegans). Planarians provide an intermediate model. They are the earliest extant animal with a primitive brain-like structure and are a convenient model of mammalian behavioral endpoints and drug-induced effects. The purpose of the present study was to determine if a quantitative measure of presumptive feeding behavior could be visualized using an imaging technique. Colored food pellets were prepared and in some experiments, plasmid-delivered green fluorescent protein was added. Both visible and fluorescence microscopy displayed clear indication of internalization of the red dye and localization to the digestive system. This new methodology establishes a convenient way to study normal physiological feeding behavior as well as modifications induced by drugs or other exogenous substances.