TITLE:
Development of a Behavioral and Imaging Model of Feeding in Planarians
AUTHORS:
Robert B. Raffa, Christopher S. Tallarida, Shan R. Patel, Scott M. Rawls, Evgeny Krynetskiy
KEYWORDS:
Feeding; Imaging; Fluorescence; GFP; Planarians
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.3 No.3,
July
5,
2012
ABSTRACT: Excess weight is a major risk factor for type-2 diabetes, cardiovascular disease, and other comorbidities. Animal models of feeding provide insight into the problem and provide a means for the discovery and evaluation of pharmacotherapeutic treatment. Mammalian models are the most commonly used, but recently non-mammalian models have been utilized (e.g., C. elegans). Planarians provide an intermediate model. They are the earliest extant animal with a primitive brain-like structure and are a convenient model of mammalian behavioral endpoints and drug-induced effects. The purpose of the present study was to determine if a quantitative measure of presumptive feeding behavior could be visualized using an imaging technique. Colored food pellets were prepared and in some experiments, plasmid-delivered green fluorescent protein was added. Both visible and fluorescence microscopy displayed clear indication of internalization of the red dye and localization to the digestive system. This new methodology establishes a convenient way to study normal physiological feeding behavior as well as modifications induced by drugs or other exogenous substances.