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X. X. Xu, R. Fu, S. W. Chen, et al., “Ezetimibe Analogs with a Reorganized Azetidinone Ring: Design, Synthesis, and Evaluation of Cholesterol Absorption Inhibitions,” Bioorganic & Medicinal Chemistry Letter, Vol. 17, No. 1, 2007, pp. 101-104. doi:10.1016/j.bmcl.2006.09.078

has been cited by the following article:

• TITLE: Development and Validation of Chiral HPLC Method for Identification and Quantification of (R)-Enantiomer in Ezetimibe

  AUTHORS: Kameswara Rao Chimalakonda, Venkatanarayana Gudala, Madhusudhan Gutta, Srinivasulu Polisetty, Sai Venkata Srinivas Koduri

  KEYWORDS: Ezetimibe; (R)-Enantiomer; Identification; Quantification and Validation

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.3 No.7, July 17, 2012

  ABSTRACT: A normal phase enantioselective high performance liquid chromatographic method was developed for enantiomeric resolution of Ezetimibe it reduces the overall delivery of cholesterol to the liver. The enantiomers of Ezetimibe were resolved on a Chiral Pak AS-H (250 × 4.6 mm, 5 μm) column using a mobile phase system containing n-Hexane, etha-nol, 2-Propanol and Trifloroacetic acid (84:12:4:0.1 v/v). The resolution between enantiomers was found to be more than 2.0. The limit of detection and limit of quantification of (R)-enantiomer were found to be 0.2 μg/mL and 0.5 μg/mL, respectively, for 10 μL injection volume. The sample solution and mobile phase were found to be stable for at least 48 h. The final optimized method was successfully applied to separate (R)-enantiomer from Ezetimibe and was proven to be reproducible and accurate for the quantitative determination of (R)-enantiomer in bulk drugs.