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Martínez-Espín, E., Martínez-González, L.J., álvarez, J.C., Roby, R.K. and Lorente, J.A. (2009) Forensic strategies used for DNA extraction of ancient and degraded museum sturgeon specimens. In: Carmona, et al., Ed., Biology, Conservation and Sustainable Development of Sturgeons, Fish & Fisheries, Series 29, Springer, 85- 96.

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• TITLE: Determining the specific status of the Iberian sturgeons by means genetic analyses of old specimens

  AUTHORS: Francisca Robles, Belén Cano-Roldán, Carmelo Ruiz Rejón, Luís Javier Martínez-González, María Jesús Álvarez-Cubero, José Antonio Lorente, José Antonio Riquelme Cantal, Pedro Aguayo de Hoyos, Javier Carrasco Rus, Miguel Cortés Sánchez, María Dolores Simón Vallejo, Manuel Ruiz Rejón, Roberto de la Herrán

  KEYWORDS: Iberian Sturgeons; A. naccarii; A. sturio; Ancient DNA; Genetic Identification; Molecular Markers.

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.1 No.3, August 16, 2010

  ABSTRACT: To clarify the species status of sturgeon from rivers of the Iberian Peninsula, eight molecular markers (4 nuclear and 4 mitochondrial) have been analysed in different specimens from historical museum samples and prehistoric samples from archaeological sites. These analyses indicate that one of these specimens (UGP captured in the Guadalquivir River in the 19th century) is A. sturio, based on all the eight molecular markers, four of them used from the first time in this study. In previous analyses based on 5 genetic markers, our group assigned two specimens captured in this river in the 1970-80s (EBD8173 and EBD8401) to the species A. naccarii, suggesting the presence of this species in the Iberian Peninsula. In this work, this conclusion is drawn after successfully obtaining a mitochondrial marker in a very old scute from a prehistoric site (Acinipo, about 1500 BC, from the Guadalquivir River basin). On the other hand, in the specimen EBD8174 captured in the Guadalquivir in 1975, we have obtained two new mitochondrial markers confirming that it can be considered A. sturio for all the mitochondrial markers, but nuclear ones identify it as A. naccarii. Finally, two very old samples (Nerja E-VI and Nerja N/62-63) were not successfully characterized by any molecular markers. Some aspects and consequences of our results are discussed, such as the origin of the “mosaic” specimen EBD8174 and, above all, the native status of A. naccarii in historic and prehistoric times in the southern Iberian Peninsula.