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Hernould, M., Suharsono, S., Zabaleta, E., Carde, J.P., Litvak, S., Araya, A. and Mouras, A. (1998) Impairment of tapetum and mitochondria in engineered male-sterile tobacco plants. Plant Molecular Biology, 36(4), 499-508.

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• TITLE: Mitochondrial comparative proteomic analysis of sterile line and its maintain line of purple cytoplasmic rice (Oryza sativa)

  AUTHORS: Lei Wei, Zhenjiang Fei, Xiaozhi Wu, Hualin Dong, Peng Zhou, Jianhua Zhang

  KEYWORDS: ATP Synthase; Comparative Proteomic Analysis; Cytoplasmic Male Sterile (CMS); Ying xiang A; Ying xiang B

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.1 No.3, August 13, 2010

  ABSTRACT: CMS/Rf systems in rice (Oryza sativa) have long been exploited for hybrid breeding to enhance productivity. Ying xiang CMS/Rf system is a new type. In this study, a mitochondrial comparative proteomic analysis of Ying xiang Sterile Line and its Maintain Line was started for a comprehensive investigation of the mitochondrial proteins’ functions in rice cytoplasmic male sterility. Mitochondria were prepared from rice shoots grown in the dark. Proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF/ MS. Using Mascot, it was found that 7 proteins were not described previously for plant mitochondria, indicating novel mitochondrial functions. 3 of them were characterized.