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A. C. Chen, S. M. Klisch, W. C. Bae, M. M. Temple, K. B. McGowan, K. R. Gratz, B. L. Schumacher and R. L. Sah, “Mechanical Characterization of Native and Tissue Engineered Cartilage,” In: M. Sabatini, F. DeCeuninck and P. Pastoureau, Eds., Cartilage and Osteoarthritis, Humana Press, Totowa, 2004, pp. 157-190. doi:10.1385/1-59259-821-8:157

has been cited by the following article:

• TITLE: Direct Visualisation of the Depth-Dependent Mechanical Properties of Full-Thickness Articular Cartilage

  AUTHORS: Matthew Szarko, Yang Xia

  KEYWORDS: Depth-Dependent Properties; Articular Cartilage; Cartilage Mechanics

  JOURNAL NAME: Open Journal of Orthopedics, Vol.2 No.2, June 22, 2012

  ABSTRACT: Objective: The structural anisotropy of articular cartilage controls its deformation response. As proteoglycans and collagen vary with depth, simple uniaxial compression results in inhomogeneous deformation with distinct depth-dependent mechanical properties. Investigations into depth-dependent mechanical properties of articular cartilage have previously required tissue modification after specimen isolation. Such modifications include histological processes, freezing, subchondral bone removal, and fluorescent staining that may alter the tissue, limiting in vivo applicability. Design: Using a custom tissue-sectioning device, 0.1 mm thick unfixed, unstained, osetochondral samples were obtained. A customized apparatus loaded samples to 12.5%, 24%, and 29% compression in under a microscope with 10× magnification. Equilibrium load was measured after stress relaxation. Intra-tissue displacement was measured by tracing groups of cells between the different compression levels using a digital imaging program. Cell distance from the subchondral bone was measured to identify intratissue displacement and calculate strain. Results: The results reveal that stress levels and intratissue displacement increased with greater tissue compression (p p in vivo conditions and may provide an important method for analyzing the coordinated changes in cartilage composition and function due to ageing and disease.