TITLE:
Sterilization of Hibiscus rosa-sinensis L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on In Vitro Direct Regeneration
AUTHORS:
Chew Tiong Dar, Janna Ong Abdullah, Parameswari Namasivayam, Siti Habsah Roowi
KEYWORDS:
Hibiscus rosa-sinensis L.; Murashige and Skoog Medium; Axillary Bud; Sucrose; Organic Ingredient; Activated Charcoal
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.3 No.6,
June
21,
2012
ABSTRACT: This paper reports on the effects of organic ingredients in facilitating direct shoot regeneration from nodal explants of Hibiscus rosa-sinensis L. This paper also compares the sterilization conditions for 3 types of explants (node, internode, and shoot tip) harvested from an open field. The optimized sterilization conditions for the explants were 40% Clorox- 20 min exposure, 10% Clorox-15 min exposure, and 5% Clorox-40 min exposure for the node, internode and shoot tip, respectively. In the direct shoot regeneration using the nodal explants, we found MS medium containing 40 g/L sucrose, 0.3% (w/v) activated charcoal, and supplementations with myo-inositol, thiamine and nicotinic acid were suitable. The in vitro shoot survival rate was 30% with a mean leaf numbers of 2.68 produced, and a mean leaf length of 1.71 cm achieved after 5 weeks of culture on the modified medium.