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Hill, P.C., Brookes, R.H., Fox, A., Fielding, K., Jeffries, D.J., Jackson-Sillah, D., Lugos, M.D., Owiafe, P.K., Donkor, S.A., Hammond, A.S., Out, J.K., Corrah, T., Adegbola, R.A. and McAdam, K.P. (2004) Large-scale evaluation of enzyme-linked immunospot assay and skin test for diagnosis of Mycobacterium tuberculosis infection against a gradient of exposure in the Gambia. Clinical Infectious Diseases, 38, 966-973. doi:10.1086/382362

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• TITLE: Interferon-gamma ELISPOT for the screening and diagnosis of latent tuberculosis infection in healthy population of China

  AUTHORS: Yi Peng, Fangfang Yang, Lin Zhou, Meigui Zhao, Yan Li, Yuhong Huang, Juan Wang, Lirong Huang, Dan Xie, Zhiquan Tu, Weiling Lin, Jinhong Liu, Qiu Zhong, Xiaomin Lai

  KEYWORDS: Latent TB Infection (LTBI); Detection; IFN-γ ELISPOT; Healthy Population of China

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.3 No.3, June 21, 2012

  ABSTRACT: The aim of this study was to analyze the combination of three kinds of in-house IFN-γ ELISPOT using peptide A53 and peptide mixtures (E6 + E7 and E6 + E7 + C14) with tuberculin skin test (TST) to detect latent TB infection (LTBI) in China. A total of 788 healthy people were recruited and analyzed by three kinds of IFN-g ELISPOT, 581 of them had TST results, of which 147 samples were also compared with the T- SPOT.TB test. The positive detection rates for T- SPOT.TB and three kinds of IFN-γ ELISPOT with A53, E6 + E7 and E6 + E7 + C14 were 14.28% (21/147), 29.43% (171/581), 23.24% (135/581) and 28.40% (165/581), respectively. These results were significantly lower than the positive TST results, which were positive in 82.99% (122/147) and 75.73% (440/ 581), respectively. The positive detection rates of three kinds of IFN-γ ELISPOT (31.60%, 26.65% and 32.11% in 788 cases, respectively) could better reflect over 40.00% of Mycobacterium tuberculosis (MTB) infection rate in China. Detection rates between contacts and non-contacts by three kinds of IFN-γ ELIS-POT were not significantly different (p > 0.05). It can be seen that the three kinds of in-house IFN-γ ELIS- POT might be used as a complementary tool of T- SPOT.TB for detecting LTBI in the healthy population of China.