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Ochiai, M., Yamamoto, A., Kataoka, M., Toyoizumi, H., Arakawa, Y. and Horiuchi, Y. (2007) A quantitative in vitro assay to detect biological activity of endotoxin using rabbit peripheral blood. Proceedings of the 6th World Congress on Alternative and Animal Use in the Life Science, 14(3), 641645.

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• TITLE: Pyrogenicity of hyaluronic acid hydrogel crosslinked by divinyl sulfone for soft tissue augmentation

  AUTHORS: JinTae Kim, JaeHa Choi, DeukYong Lee

  KEYWORDS: Hyaluronic Acid Hydrogel; Injectable Dermal Filler; Pryogenicity; Endotoxin; Interfering Factor; Soft Tissue Augmentation

  JOURNAL NAME: Natural Science, Vol.2 No.7, July 30, 2010

  ABSTRACT: Hyaluronic acid hydrogels (HAHs) were synthesized by immersing the microbeads in phosphate buffered saline solution to assess short term biocompatibility of the gels by means of the rabbit pyrogen test and the bacterial endotoxin test. The rise in body temperature of 3 male New Zealand white rabbits weighing about 2~3 kg (12~16 weeks old) following intravenous injection of the test article (10 mL/kg) was mo nitored at 30 min intervals in 3 h to examine the pyrogenicity. No rabbits showed an individual rise in temperature of 0.5oC or more above its respective control temperature. The temperature rises of the rabbits after injection were 0.12oC, 0.13oC, and 0.18oC, respectively, sugge sting that HAH meets the requirements for the absence of pyrogens. The bacterial endotoxin test revealed that the concentration of endoto xins required to cause the lysate to clot under standard conditions was