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R. M. Carr, U. J. Blumenthal and D. D. Mara, “Guidelines for the Safe Use of Wastewater in Agriculture: Revisiting WHO Guidelines,” Water Science and Technology, Vol. 50, No. 2, 2004, pp. 31-38.

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• TITLE: Molecular Assessment of 16S-23S rDNA Internal Transcribed Spacer Length Polymorphism of Aeromonas hydrophila

  AUTHORS: Vijai Singh, Indra Mani, Dharmendra Kumar Chaudhary

  KEYWORDS: Aeromonas hydrophila; Diseases; ITS; tRNA-Glu; Molecular Marker

  JOURNAL NAME: Advances in Microbiology, Vol.2 No.2, June 13, 2012

  ABSTRACT: Aeromonas hydrophila is an important bacterial pathogen which causes the hemorrhagic septicemia in fishes, amphibians and humans. Genetic relationships of diverse isolates of A. hydrophila were recovered from fish and water sources. These isolates were investigated by flanked region of 16S and 23S Ribosomal DNA (rDNA) internal transcribed spacer (ITS). Here we analyzed polymorphism of PCR-amplified 16S-23S rDNA ITS and their revealed band pattern consisting of one to four DNA fragments. The fragment size ranged from 500 to 1000 bp. The DNA band patterns revealed a considerable genetic diversity in interaspecies. The 750 bp size of band was common in all isolates of A. hydrophila except one Isolates AH21. The tRNA-Glu sequences were identified from 750 bp size of ITS region that could be used as strain level potential genotypic markers.