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Genuth, S., Sun, W., Cleary, P., Sell, D.R., Dahms, W., Malone, J., Sivitz, W., and Monnier, V.M. (2005) Glycation and carboxymethyllysine levels in skin collagen predict the risk of future 10-year progression of diabetic retinopathy and nephropathy in the diabetes control and complications trial and epidemiology of diabetes interventions and complications participants with type 1 diabetes. Diabetes, 54, 3103-3111. doi:10.2337/diabetes.54.11.3103

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• TITLE: Fluorescent advanced glycation end-products (ages) detected by spectro-photofluorimetry, as a screening tool to detect diabetic microvascular complications

  AUTHORS: Maria Pia de la Maza, Fernando Garrido, Nadia Escalante, Laura Leiva, Gladys Barrera, Sylvia Schnitzler, Mario Zanolli, Juan Verdaguer, Sandra Hirsch, Natallia Jara, Daniel Bunout

  KEYWORDS: Advanced Glycation End-Products; Ages; Microangiopathy; Microvascular Complications

  JOURNAL NAME: Journal of Diabetes Mellitus, Vol.2 No.2, May 25, 2012

  ABSTRACT: BACKGROUND: Advanced glycation end-products (AGEs) are one of the mechanisms related to diabetic vascular complications. However, since AGEs are multiple and heterogeneous moieties, there is no universally accepted method to measure them for clinical purposes. The aim of this work was to study the utility of a simple fluorimetric assay as predictor of complications. METHODS: Blood samples from 102 type 2 diabetic patients were obtained to assess glucose, glycosylated haemoglobin, creatinine, lipoproteins and C Reactive Protein (CRP), fluorescent AGES by spectrophotofluorimetry and non-fluorescent AGEs by measurement of N(ε)-carboxymethyl-Lysine (CML) using an ELISA kit in a subsample of 82 patients. Urinary fluorescent AGEs, albumin and creatinine were also measured in a morning urine sample. Microvascular complications were studied by ophthalmologic examination, albuminuria and peripheral nerve conduction velocity. RESULTS: Patients without microvascular complications had significantly lower levels of both serum and urinary AGEs. CML was associated with retinopathy. Multiple regression analysis confirmed that AGEs, length of diabetes and glycosylated haemoglobin were all variables associated with diabetic complications, in this sample. CONCLUSIONS: A simple fluorimetric assay to measure low molecular weight fluorescent AGEs, and CML could be employed as screening tools to predict diabetic complications, at a primary care setting. AGEs should probably be considered as another therapeutic target in diabetes management.