TITLE:
Effect of Recombinant Human Erythropoietin on Survivin Expression in Brain Tissues after Traumatic Brain Injury in Rats
AUTHORS:
Peng Lei, Longfeng Peng, Xingchao Zhang
KEYWORDS:
Recombinant Human Erythropoietin (r-HuEPO); Brain Injury; Survivin; NF-κB; Apoptosis
JOURNAL NAME:
Surgical Science,
Vol.3 No.5,
May
25,
2012
ABSTRACT: Objective: To explore the regulative effect on Survivin of r-HuEPO after traumatic brain injury (TBI) in rats, and understand the neuroprotection mechanisms of r-HuEPO. Methods: Seventy-eight adult Wistar rats were randomly divided into sham operation group (n = 6), TBI group (n = 36) and r-HuEPO group (n = 36). The experimental TBI model was created by Feeney’s method. Samples were obtained after injury for measuring apoptosis of cells by Epics XL Flow Cytometer. Immunochemical method was performed for inspection of expressions of Survivin and NF-κB proteins. Results: Compared to the sham group, the number of apoptotic cells and Survivin, NF-κB immunopositive cells was significantly increased in the injured brain after TBI (P