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S. S. LateEf, S. Gupta, L. P. Jayathlilaka, S. Krishnanchettia, J. S. Huanq and B. S. Lee, “An Improved Protocol for Coupling Synthetic Peptides to Carrier Proteins for Antibody Production Using DMF to Solubilize Peptides,” Journal of Biomolecular Techniques, Vol. 18, No. 3, 2007, pp. 173-176.

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• TITLE: A Novel Immunological Technique for Detection of Gramicidin (S) Producing Bacteria

  AUTHORS: Fouad Houssein Kamel

  KEYWORDS: Gramicidin; Serological Kit; Immuno-complex

  JOURNAL NAME: Green and Sustainable Chemistry, Vol.2 No.2, May 11, 2012

  ABSTRACT: A serological kit was prepared for the first time to detect bacteria that produce Gramicidin (S). Since, an immunocomplex of antibiotic Gramicidin (S) was prepared followed by immunonization of laboratory animales to obtain specific antibodies in their serum. Serial dilutions of the prepared antibodies were tested directly against samples of bacteria and compared with control samples, which include bacteria that produce Gramicidin (S) (as a positive control) and the bacteria that does not produce Gramicidin (S) (as a negative control) depending slide method after the incubation of bacteria in a suitable media for 3 - 4 hours at 37°C. The prepared kites were evaluated in reference Laboratories and they proved to be highly sensitive (100%), specific (100%), more economic and reliable procedure, in addition to its stability for more than one year. It was the first time to prepare a kit for detection of bacteria which produce Gramicidin (S) directly within few minutes.