TITLE:
Acute Toxicity and Analgesic Effect of Pentaclethra eetveldeana De Wild & Th. Dur Leaves Aqueous Extract (Fabaceaee)
AUTHORS:
Gelase Fredy Nsonde Ntandou, Guesdon Treich Delacour Nombo Lassy, Sarah Luce Boumba, Victor N’Goka, Stevie Sharon Landou Pandzou, Djosmy Rolf Mban, Dieurci Prince-Delou Louzolo, Justine Rosa Elisa Ahoudji, Ghilson Sinclair Makemba Nkounkou, Veraldo Malanda Bwana-Mukuba
KEYWORDS:
Pentaclethra eetveldeana, Acute Toxicity, Analgesic, Mechanism of Action
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.14 No.11,
November
12,
2026
ABSTRACT: Toxicological and biological studies are essential to confirm the therapeutic or nutritional effects and assess the risks associated with the use of medicinal plants. The aim of this work was to study the acute toxicity and evaluate the analgesic and anti-inflammatory effects of the aqueous extract of P. eetveldeana leaves. The anti-inflammatory effect was assessed using the carrageenan edema induction test. The acute toxicity study, conducted according to OECD guideline no. 423 (2001), showed that this extract is mildly toxic, with an estimated LD50 of 2375 mg/kg. Exploratory behavioral and mobility disturbances, associated with dyspnea, were observed at doses of 2000, 3000, and 5000 mg/kg. At doses of 250 and 500 mg/kg, the aqueous extract of P. eetveldeana did not significantly reduce rat paw edema induced by 1% carrageenan. In the acetic acid-induced pain test, the aqueous extract showed a significant analgesic effect only at the 500 mg/kg dose. In contrast, in the hot water tail immersion pain test, a significant analgesic effect was observed at both 250 and 500 mg/kg doses. Furthermore, naloxone inhibited the analgesic effect of the extract only at the 250 mg/kg dose, in the hot water tail immersion pain test. The aqueous extract of P. eetveldeana exerts an analgesic effect involving both two mechanisms of action, central and peripheral.