TITLE:
Experimental Teaching Design of Exploring the Protective Effect of Vegetable Active Components on DNA via Fluorescent Probe Method under the Background of New-Medical-Science Education
AUTHORS:
Ao Chen, Hairong Zhang, Qingqing Deng, Zonglin Yang, Jie Zhang
KEYWORDS:
New-Medical-Science Education, Fluorescent Probe, Antioxidation, Exploratory Experiment
JOURNAL NAME:
Open Journal of Applied Sciences,
Vol.16 No.9,
September
18,
2026
ABSTRACT: To meet the requirements of the Healthy China Initiative and the development of new-medical-science education, and implement the health concept of shifting from treatment-oriented mode to wholelifecycle health management, this paper integrates the concept of clinical preventive medicine into biochemistry experimental teaching, and designs an exploratory experiment themed on the protective effect of vegetable active components on DNA. The ethidium bromide fluorescent probe method was adopted to characterize EB-DNA fluorescence modulation triggered by flavonoids, polyphenols and vitamin C extracts from 11 common vegetables. Through active-component extraction, content determination, fluorescence spectrum analysis and action constant calculation, students are guided to build interdisciplinary thinking integrating medicine and chemistry, understand the role of daily food in disease prevention and treatment, and explore the antioxidant and potential anti-cancer mechanisms of vegetables. Breaking the barriers between basic experiments and clinical medicine, this experiment effectively improves students’ scientificresearch thinking and clinical practical literacy, and highlights the educational characteristics of new-medical-science education featured with humanistic care and whole-life-cycle health management.