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Rodrigues, L.L.V., Moura, Y.B.F., Viana, J.V.D.S., Oliveira, L.R.M.D., Praxedes, É.A., Vieira Neto, J.D.B., et al. (2023) Full Confluency, Serum Starvation, and Roscovitine for Inducing Arrest in the G₀/G₁ Phase of the Cell Cycle in Puma Skin-Derived Fibroblast Lines. Animal Reproduction, 20, e20230017.
https://doi.org/10.1590/1984-3143-ar2023-0017

has been cited by the following article:

• TITLE: Optimization of Serum Concentration, Synchronization Duration, and Cell Confluence on the Cell-Cycle Synchronization Efficiency in Thanh Chương Buffalo Fibroblasts

  AUTHORS: Huong Thi Thu Vu, Au Thi Hoang, Yen Thi Kim Pham, Dat Van Le, Lan Anh Thi Nguyen, Hieu Trung Phan, Duc Viet Phan, Huong Le Thi Nguyen, Nguyen Thi Nhien, Nguyen Thi Van Anh, Lan Doan Pham, Van Khanh Nguyen

  KEYWORDS: Buffalo Fibroblast, Cycle Synchronisation, Serum Concentration, Synchronization Duration, Cell Confluence

  JOURNAL NAME: Open Journal of Animal Sciences, Vol.16 No.4, September 15, 2026

  ABSTRACT: This study aimed to optimize serum concentration, synchronization duration, and cell confluence to improve cell-cycle synchronization efficiency in Thanh Chương buffalo fibroblasts. The proportion of nucleated fibroblasts in the G0/G1 phase was the highest at a serum concentration of 0.3% (81.26%) and the lowest in the control group (61.44%), with the difference being statistically significant (P 0.05). The proportion of fibroblasts in the G0/G1 phase was significantly higher in the 96-hour and 120-hour synchronization groups compared with the 24-hour, 48-hour, and 72-hour groups (P 0.05). Similarly, fibroblasts at 80% and 100% confluence exhibited a significantly higher proportion in the G0/G1 phase compared with those at 60% confluence (86.32% and 85.91% vs 70.56%, respectively; P