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E. Villafranca, Y. Okruzhnov, M. A. Dominguez, J. Garcia-Foncillas, I. Azinovic, E. Martinez, et al., “Polymorphisms of the Repeated Sequences in the Enhancer Region of the Thymidylate Synthase Gene Promoter May Predict Downstaging after Preoperative Chemoradiation in Rectal Cancer,” Journal of Clinical Oncology, Vol. 19, No. 6, 2001, pp. 1779-1786.

has been cited by the following article:

• TITLE: Thymidylate Synthase 5’ UTR Polymorphic Allele Distribution in South Florida Population

  AUTHORS: Hiba Hammad, Malancha Sarkar, Pochi R. Subbarayan, Bach Ardalan

  KEYWORDS: TYMS Enhancer Region; Polymorphic Repeats; Translation Regulation; 5-FU Resistance; Colorectal Cancer

  JOURNAL NAME: Journal of Cancer Therapy, Vol.3 No.2, April 24, 2012

  ABSTRACT: Thymidylate synthase (TS; TYMS) is a pivotal enzyme in the DNA synthesis pathway. The 5’UTR of TYMS gene has a polymorphic 28 bp segment. Presence of two or three repeats of this unique 28 bp sequence is common. A distinct population distribution pattern for this polymorphic trait among different racial groups had been reported. We analyzed TYMS genotype in the peripheral blood mononucleocytes (PBMC) of 74 individuals in the South Florida region of the United States of America. The number of 28 bp repeats in the 5’ UTR was determined by PCR followed by agarose gel electrophoresis. The distribution of the three different genotypes was found to be 35.1% for 2R/2R, 39.2% for 2R/3R and 24.3% for 3R/3R. One individual was detected with 3R/4R genotype. Functional analyses associated homozygous for three repeats (3R/3R) to higher TYMS expression and therefore poor prognosis to chemotherapy. The other possible genotypes viz 2R/2R or 2R/3R is proposed to have better prognosis. However, there are reports that challenge this observation.