TITLE:
Prevalence of Malaria and Diversity of pfmdr1 Gene Polymorphisms among Plasmodium falciparum Isolates in Daloa, Western C?te d’Ivoire
AUTHORS:
Bernardin Ahouty Ahouty, Siriki Simaro, Anique Ahou Gbotto, Ouangbo Yaya Ouattara, Lydia Karmelle Ornella Dago, Georges Bohoussou Kassi, Abla Edwige Sokouri, Yao Mathurin Koffi, Thomas Konan Konan, Mathurin N’Goran Koffi
KEYWORDS:
Malaria, Plasmodium falciparum, pfmdr1 Gene, Codon, Daloa, C?te d’Ivoire
JOURNAL NAME:
Open Journal of Genetics,
Vol.16 No.3,
July
31,
2026
ABSTRACT: Resistance of Plasmodium falciparum to antimalarial drugs remains a major public health concern in endemic regions. Polymorphisms in the pfmdr1 gene, particularly N86Y and Y184F, have been implicated in modulating parasite susceptibility to artemisinin-based combination therapies (ACTs). This study aimed to investigate the molecular epidemiology of P. falciparum and the distribution of pfmdr1 polymorphisms in Daloa, western Côte d’Ivoire. A cross-sectional study was conducted among individuals with suspected clinical malaria at the Regional Center for Mutuality and Social Works in Schools (CREMOSS) of Daloa. Blood samples were collected for parasitological and molecular analyses. Detection of P. falciparum and genotyping of pfmdr1 polymorphisms were performed using standard molecular techniques. Associations between infection status, genetic markers, and sociodemographic variables were also assessed. The overall molecular prevalence of P. falciparum was 50.27%, indicating sustained transmission within the study population. No significant association was observed between infection status and sex, whereas age influenced infection risk, with children aged 6 - 15 years being the most affected group. Analysis of the pfmdr1 gene revealed a high prevalence of the mutant alleles 86Y and 184F, with a marked predominance of the double mutant haplotype 86Y-184F (65.00 %). The wild-type haplotype N86-Y184 was infrequent, while the 86Y-Y184 haplotype was nearly absent. No significant association was found between pfmdr1 polymorphisms and sociodemographic variables. The high prevalence of pfmdr1 mutant alleles and the predominance of the 86Y-184F haplotype suggest ongoing selection pressure, likely associated with the use of antimalarial drugs within the study population. The low frequency of wild-type genotypes may reflect a progressive decline in drug-sensitive parasites. These findings highlight the need for continuous molecular surveillance to monitor changes in ACT efficacy and guide malaria control strategies.